Lentivirus vector
The lentiviral vector system is a very efficient vector tool for the stable integration of foreign genes into mammalian cells.
In addition to conventional plasmid transfection, this system is currently one of the most commonly used methods to tranfer foreign genes into mammalian cells. Due to the flexibility of target gene and promoter selection and the universality of the transfected cell types, the lentiviral vector system is a popular foreign gene expression system.
Lentiviral vectors are derived from the human immunodeficiency virus（HIV） and belong to the retroviral family. The wild-type lentiviral genome is a linear double plus-strand RNA.
The lentiviral recombinant vector construct was transfected into packaging cells together with the helper plasmid. In packaging cells, DNA fragments located between two long terminal repeats (LTR) are transcribed into RNA and packaged into viral particles by viral proteins expressed from helper plasmids. The packaged live virus will be released into the supernatant, which can directly collect or further concentrate the virus for transfected target cells.
When viruses transduce target cells, viral RNA released into the host cell is reverse transcribed into double-stranded DNA with the help of reverse transcriptase, which is then randomly integrated into the genome of the host cell. In the viral vector, the DNA fragments located in both LTR and the viral genome are stably integrated into the genome of the target cell.
For more information on lentiviral gene expression vectors, please refer to the following literature.
In addition to conventional plasmid transfection, this system is currently one of the most commonly used methods to tranfer foreign genes into mammalian cells. Due to the flexibility of target gene and promoter selection and the universality of the transfected cell types, the lentiviral vector system is a popular foreign gene expression system.
Lentiviral vectors are derived from the human immunodeficiency virus（HIV） and belong to the retroviral family. The wild-type lentiviral genome is a linear double plus-strand RNA.
The lentiviral recombinant vector construct was transfected into packaging cells together with the helper plasmid. In packaging cells, DNA fragments located between two long terminal repeats (LTR) are transcribed into RNA and packaged into viral particles by viral proteins expressed from helper plasmids. The packaged live virus will be released into the supernatant, which can directly collect or further concentrate the virus for transfected target cells.
When viruses transduce target cells, viral RNA released into the host cell is reverse transcribed into double-stranded DNA with the help of reverse transcriptase, which is then randomly integrated into the genome of the host cell. In the viral vector, the DNA fragments located in both LTR and the viral genome are stably integrated into the genome of the target cell.
For more information on lentiviral gene expression vectors, please refer to the following literature.
| Reference Documentation | Theme |
| J Virol. 72:8463 (1998) | The 3rd generation lentivirus vectors |
| J Virol. 72:9873 (1998) | Self-inactivating lentivirus vectors |
| Science. 272:263 (1996) | Transduction of non-dividing cells by lentivirus vectors |
| Curr Gene Ther. 5:387 (2005) | Tropism of lentiviral vectors |
| J Virol. 77:4685 (2003) | Impact of cPPT to lentivirus vector transduction |
| J Virol. 73:2886 (1999) | WPRE enhances the expression of transgenes |
| Nat Protoc. 1:241 (2006) | Production and purification of lentiviral vectors |
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